Part:BBa_K1356002:Design

Dispersin B gene with DsbA signalling sequence for export from N. multiformis

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1051
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The DsbA signaling sequence was attached to the Dispersin B gene using PCR. Due to the length of the forward primer, overlap extension PCR was used to piece it together prior to attaching it to the Dispersin Gene.

Source

The Dispersin B DNA was aquired from part BBa_K1195000. The signaling sequence was simply ordered along with our primers.

Dispersin B was originally discovered in Aggregatibacter actinomycetemcomitans, a pathogenic bacteria which causes gum disease. The bacteria uses excreted Dispersin B protein to disperse it's own biofilm formations, and enable it to spread to other locations (Kaplan, Ragunath,Ramasubbu,& Fine, 2003).

References

Kaplan, J. B., Ragunath, C., Ramasubbu, N., & Fine, D. H. (2003). Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous Hexosaminidase Activity. Journal of Bacteriology. doi:10.1128/JB.185.16.4693-4698.2003